CytoTrace Green CMFDA
CytoTrace Green CMFDA (Ex-Max 499 nm/Em-Max 514 nm)
CytoTrace Green CMFDA is chloromethyl (CM) derivitive of fluorescein diacetate which loads passively into live cells where its retained by the action of intracellular esterases. The chloromethyl crosslinks with intracellular thiol side chains for a moderate level of retention.
Excitation & emission spectra
Key facts
- Excitation maximum
- 499 nm
- Emission maximum
- 514 nm
- Optimal laser line
- 488
- Optimal filter
- 515/30
- Commercial antibodies
- 1 from 1 supplier
CytoTrace Green CMFDA has an excitation maximum at 499 nm and an emission maximum at 514 nm, a Stokes shift of 15 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is CytoTrace Green CMFDA?
- CytoTrace Green CMFDA is chloromethyl (CM) derivitive of fluorescein diacetate which loads passively into live cells where its retained by the action of intracellular esterases. The chloromethyl crosslinks with intracellular thiol side chains for a moderate level of retention.
- What laser excites CytoTrace Green CMFDA?
- CytoTrace Green CMFDA is optimally excited by the 488 laser line. Its excitation maximum is 499 nm.
- What filter should I use to detect CytoTrace Green CMFDA?
- A 515/30 filter captures the peak of the CytoTrace Green CMFDA emission near 514 nm with minimal spillover.
- How many antibodies are available conjugated to CytoTrace Green CMFDA?
- FluoroFinder tracks 1 commercially available CytoTrace Green CMFDA conjugates, searchable by target, clone, host and application.